Malaria is caused by parasites transmitted by mosquito bite. The most dangerous form of malaria is malaria tropica. Left untreated, it is fatal in most cases. An early diagnosis is vital for the timely and right kind of therapy. There are five different types of parasites at the origin of the disease. Plasmodium falciparum is causing malaria tropica and it is particularly harmful for infants and pregnant women in the African sub-Saharan region.
To diagnose malaria with conventional diagnostic tools and to identify the parasite causing the infection takes experienced and well-trained personnel and a well-equipped laboratory. Both are scarce in Africa or even anywhere else outside specialized health care centers.
That is the reason why the NanoFRET project partners come together to develop the whole blood rapid diagnostic test to detect just this special type of parasite. Funding is granted by the German Federal Ministry of Education and Research (BMBF) within the framework of the VIP+ program (“Validation of the innovation potential of scientific research”).
In the body, infectious germs produce large amounts of specific proteins that accumulate in the blood. These proteins will be detected by a new tool developed by the project consortium led by the project coordinator Dr. Rolf Fendel (University of Tübingen). At the Fraunhofer IME, the team around Dr. Torsten Klockenbring sets out to develop antibodies able to identify proteins from the malaria parasite. These antibodies are coupled to novel fluorescent nanoparticles, which Dr. Sofia Dembski and her “Theranostik” team – part of the Translational Center Regenerative Therapies –developed at the Fraunhofer ISC.
The detection of the pathogen in a blood sample will be based on a special technique (time-resolved fluorescence resonance energy transfer (TR-FRET) in combination with the antibodies and the nanoparticles.
However, there is one challenge to overcome: The fluorescent properties of the nanoparticles have to be adapted so that the autofluorescence of blood can not affect the result.
Testing will take place with samples from malaria patients and a non-infected control group. Samples will be gathered, characterized and used to define the test parameters. The study will be performed by Dr. Andrea Kreidenweiss from the Institute of Tropical Medicine (University of Tübingen) at the Centre de Recherches Médicales de Lambaréné (CERMEL) located in Gabon, a collaboration partner of long standing.
In a next step upon establishing a reliable method, it will be tested in a diagnostic study at the CERMEL. The evaluation will consider sensitivity, specificity, and practicability under real conditions. If the test proves to be suitable, the project partners will design a prototype kit. The test kit must be producible at low cost and should enable an early diagnosis with malaria tropica.
The project is planned to be completed by late 2019. If successful, the project partners will adapt the test method to other infectious diseases and then find industry partners to develop the next generation of rapid diagnostic tools.
Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany
Institute of Tropical Medicine at the University of Tübingen, Germany
Fraunhofer Institute for Silicate Research ISC, Würzburg, Germany