In their study, the researchers showed that the opening and closing of ion channels literally "hangs by a thread". This protein thread or filament, as Dr. Hu and Professor Lewin demonstrated, is synthesized by the mechanosensitive endings of cutaneous neurons and is probably an integral part of the mechanosensitive mechanism.
The thread is firmly tethered in the extracellular matrix (ECM), the connective protein "glue" that helps to hold cells together. However, the filament is located so close to the mechanosensitive ion channels that it can probably directly open them. The filaments were found to be 100 nanometers (nm) long (1 nanometer is equivalent to one billionth of a meter) and may link the ion channels of the cell membrane to the ECM at mechanosensitive sensory endings of the skin in mice.
The researchers demonstrated both with neuronal cultures and experiments using the isolated skin with receptors attached that the opening of mechanosensitive ion channels upon slight touch requires the 100nm protein filament. The stretching of sensory membranes by small mechanical stimuli does not appear to play any significant role in touch receptors.
When the researchers cleaved the filament with specific enzymes, thus cutting the link between the sensory ending and the extracellular matrix (ECM), the neurons were rendered completely insensitive to mechanical stimulation and touch. However, if the researchers waited twelve hours the filaments were again synthesized by the sensory cells and they became mechanosensitive once more.
"This means that touch can be perceived only when the protein filament is present. The filament renders the mechanosensitive ion channel highly sensitive to force and may even directly participate in opening and closing the channel " Professor Lewin explained.
However, this does not apply to the perception of mechanical pain. "Pain receptors" he emphasized, "are not dependent on this filament." According to the neurobiologists, the protein filaments may in the future be of great interest to medical research. Advancements in this area could help people whose sense of touch is impaired due to old age, improving their general well-being and mobility. There are also common syndromes where there is oversensitivity to touch, in the case of neuropathic pain where the slightest touch of feather may be perceived as painful, again accessing the tether may help in alleviating the symptoms.
*Evidence for a protein tether involved in somatic touch
Jing Hu1,2,4, Li-Yang Chiang1,2, Manuel Koch3 and Gary R. Lewin1
1Department of Neuroscience, Max-Delbrück Center for Molecular Medicine and Charité Universitätsmedizin Berlin, Robert-Rössle-Str. 10, Berlin-Buch D-13125 Germany. 3Center for Biochemistry, Department of Dermatology, and Center for Molecular Medicine Cologne, Medical Faculty, University of Cologne, D-50931, Cologne, Germany.
2These authors made an equal contribution.
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